transcriptomics — papers

BiologyNature · Oct 2018 Open access

Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris

Nicholas Schaum, Stephen R. Quake and Tony Wyss-Coray

The Tabula Muris Consortium generated a compendium of single-cell transcriptome data spanning 20 organs and tissues from the mouse Mus musculus. Both droplet-based (10x) and FACS-sorted plate-based (Smart-seq2) methods were used to profile cells, enabling characterization of cell types across the organism. The resource provides a cross-tissue reference for defining and comparing mouse cell populations.

single-cell rna-seq mouse atlas tabula muris cell types

BiologyNature Communications · Jan 2017 Open access

Massively parallel digital transcriptional profiling of single cells

Grace X. Y. Zheng, Jessica M. Terry, Phillip Belgrader and Jason H. Bielas

This paper introduced a droplet-based microfluidic platform (the 10x Genomics GemCode/Chromium system) for high-throughput single-cell RNA sequencing using barcoded gel beads. The method enables 3' digital expression profiling of thousands of cells per run at low cost. The authors profiled tens of thousands of cells, including ~68,000 PBMCs, demonstrating the ability to resolve immune cell subpopulations and detect rare cell types.

single-cell rna-seq droplet microfluidics 10x genomics transcriptomics