Massively parallel digital transcriptional profiling of single cells
This paper introduced a droplet-based microfluidic platform (the 10x Genomics GemCode/Chromium system) for high-throughput single-cell RNA sequencing using barcoded gel beads. The method enables 3' digital expression profiling of thousands of cells per run at low cost. The authors profiled tens of thousands of cells, including ~68,000 PBMCs, demonstrating the ability to resolve immune cell subpopulations and detect rare cell types.