Massively parallel digital transcriptional profiling of single cells

Grace X. Y. Zheng, Jessica M. Terry, Phillip Belgrader, Jason H. Bielas · Large author list (10x Genomics team); Zheng G.X.Y. first author, Bielas J.H. corresponding/senior. Full list includes Terry, Belgrader, Ryvkin, Bent, Wilson, Ziraldo, Wheeler, McDermott, Zhu, Gregory, Shuga, Montesclaros, Underwood, Masquelier, Nishimura, Schnall-Levin, Wyatt, Hindson C.M., Bharadwaj, Wong, Ness, Beppu, Deeg, McFarland, Loeb, Valente, Ericson, Stevens, Radich, Mikkelsen, Hindson B.J.

Published 16 January 2017 · Nature Communications · Journal article

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Summary

This paper introduced a droplet-based microfluidic platform (the 10x Genomics GemCode/Chromium system) for high-throughput single-cell RNA sequencing using barcoded gel beads. The method enables 3' digital expression profiling of thousands of cells per run at low cost. The authors profiled tens of thousands of cells, including ~68,000 PBMCs, demonstrating the ability to resolve immune cell subpopulations and detect rare cell types.

Key findings

Demonstrated a scalable droplet platform barcoding thousands to tens of thousands of single cells per experiment.
Profiled ~68,000 peripheral blood mononuclear cells and classified immune subpopulations.
Showed the method's accuracy and sensitivity, establishing a widely adopted commercial scRNA-seq workflow.

Subjects & keywords

Biology & Genetics single-cell rna-seq droplet microfluidics 10x genomics transcriptomics genomics

Cite this paper

APA

Grace X. Y. Zheng, Jessica M. Terry, Phillip Belgrader, & Jason H. Bielas [Large author list (10x Genomics team); Zheng G.X.Y. first author, Bielas J.H. corresponding/senior. Full list includes Terry, Belgrader, Ryvkin, Bent, Wilson, Ziraldo, Wheeler, McDermott, Zhu, Gregory, Shuga, Montesclaros, Underwood, Masquelier, Nishimura, Schnall-Levin, Wyatt, Hindson C.M., Bharadwaj, Wong, Ness, Beppu, Deeg, McFarland, Loeb, Valente, Ericson, Stevens, Radich, Mikkelsen, Hindson B.J.] (2017). Massively parallel digital transcriptional profiling of single cells. Nature Communications. https://doi.org/10.1038/ncomms14049

BibTeX

@article{zheng2017massively,
  author    = {Grace X. Y. Zheng and Jessica M. Terry and Phillip Belgrader and Jason H. Bielas and {Large author list (10x Genomics team); Zheng G.X.Y. first author, Bielas J.H. corresponding/senior. Full list includes Terry, Belgrader, Ryvkin, Bent, Wilson, Ziraldo, Wheeler, McDermott, Zhu, Gregory, Shuga, Montesclaros, Underwood, Masquelier, Nishimura, Schnall-Levin, Wyatt, Hindson C.M., Bharadwaj, Wong, Ness, Beppu, Deeg, McFarland, Loeb, Valente, Ericson, Stevens, Radich, Mikkelsen, Hindson B.J.}},
  title     = {Massively parallel digital transcriptional profiling of single cells},
  journal   = {Nature Communications},
  year      = {2017},
  doi       = {10.1038/ncomms14049},
  url       = {https://doi.org/10.1038/ncomms14049}
}

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