Keyword

dna sequencing

2 papers tagged “dna sequencing”

BiologyNature · Feb 2001

Initial sequencing and analysis of the human genome

Eric S. Lander, Lauren M. Linton, Bruce Birren, Chad Nusbaum and Michael C. Zody

This paper reported the results of the publicly funded Human Genome Project, presenting and making freely available a draft sequence covering the great majority of the human genome along with an initial analysis. The consortium described the broad genomic landscape—including gene content, repeat elements, GC content, and recombination rates—and estimated a surprisingly low number of protein-coding genes, on the order of roughly 30,000–40,000. The work provided a foundational reference for human biology, medicine, and evolutionary studies.

human genome genomics dna sequencing human genome project

BiologyProceedings of the National Academy of Sciences · Dec 1977 Open access

DNA sequencing with chain-terminating inhibitors

F. Sanger, S. Nicklen and A. R. Coulson

Sanger, Nicklen, and Coulson introduced a new method for determining nucleotide sequences in DNA, building on their earlier 'plus and minus' technique. The method uses 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase, generating a set of partially extended chains that can be size-separated by gel electrophoresis to read the sequence. Applied to bacteriophage φX174 DNA, the approach proved faster and more accurate than the original plus or minus method.

dna sequencing sanger sequencing molecular biology methods