Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, Feng Zhang · Full author list: Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler, Aaron J. Dy, Julia Joung, Vanessa Verdine, Nina Donghia, Nichole M. Daringer, Catherine A. Freije, Cameron Myhrvold, Roby P. Bhattacharyya, Jonathan Livny, Aviv Regev, Eugene V. Koonin, Deborah T. Hung, Pardis C. Sabeti, James J. Collins, Feng Zhang.

Published 13 April 2017 · Science · Journal article

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Summary

This paper introduced SHERLOCK, a CRISPR-based nucleic acid detection platform built on the collateral RNase activity of Cas13a (C2c2). Upon recognizing a target sequence, Cas13a indiscriminately cleaves nearby reporter RNAs; combined with isothermal amplification, this yields highly sensitive, specific detection. The authors demonstrated attomolar sensitivity and single-base discrimination, with applications in detecting viruses (Zika, dengue), bacteria, and human genotypes.

Key findings

Exploited Cas13a collateral RNase cleavage to build the SHERLOCK detection platform
Combined with isothermal amplification to reach attomolar sensitivity and single-nucleotide specificity
Detected pathogens (Zika, dengue), distinguished bacterial strains, and identified human genotypes

Subjects & keywords

Biology & Genetics cas13 diagnostics crispr nucleic acid detection sherlock

Cite this paper

APA

Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, & Feng Zhang [Full author list: Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler, Aaron J. Dy, Julia Joung, Vanessa Verdine, Nina Donghia, Nichole M. Daringer, Catherine A. Freije, Cameron Myhrvold, Roby P. Bhattacharyya, Jonathan Livny, Aviv Regev, Eugene V. Koonin, Deborah T. Hung, Pardis C. Sabeti, James J. Collins, Feng Zhang.] (2017). Nucleic acid detection with CRISPR-Cas13a/C2c2. Science. https://doi.org/10.1126/science.aam9321

BibTeX

@article{gootenberg2017nucleic,
  author    = {Jonathan S. Gootenberg and Omar O. Abudayyeh and Jeong Wook Lee and Feng Zhang and {Full author list: Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler, Aaron J. Dy, Julia Joung, Vanessa Verdine, Nina Donghia, Nichole M. Daringer, Catherine A. Freije, Cameron Myhrvold, Roby P. Bhattacharyya, Jonathan Livny, Aviv Regev, Eugene V. Koonin, Deborah T. Hung, Pardis C. Sabeti, James J. Collins, Feng Zhang.}},
  title     = {Nucleic acid detection with CRISPR-Cas13a/C2c2},
  journal   = {Science},
  year      = {2017},
  doi       = {10.1126/science.aam9321},
  url       = {https://doi.org/10.1126/science.aam9321}
}

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