Spatially resolved, highly multiplexed RNA profiling in single cells
Kok Hao Chen, Alistair N. Boettiger, Jeffrey R. Moffitt, Siyuan Wang, Xiaowei Zhuang
Summary
This paper introduces MERFISH, a single-molecule imaging method that uses error-robust barcoding combined with sequential rounds of hybridization and imaging to identify many RNA species in single cells in situ. The error-correcting binary codes allow detection and misidentification correction across thousands of transcripts. The authors imaged up to ~1,000 genes in individual human cells, mapping spatial expression and revealing gene co-variation and subcellular RNA localization.
Key findings
- Developed MERFISH using combinatorial, error-robust barcoding read out over multiple imaging rounds.
- Simultaneously profiled hundreds to ~1,000 RNA species in single cells while preserving spatial context.
- Revealed correlated gene expression groups and spatial/subcellular RNA distribution patterns.
Subjects & keywords
Cite this paper
Kok Hao Chen, Alistair N. Boettiger, Jeffrey R. Moffitt, Siyuan Wang, & Xiaowei Zhuang (2015). Spatially resolved, highly multiplexed RNA profiling in single cells. Science. https://doi.org/10.1126/science.aaa6090
@article{chen2015spatially,
author = {Kok Hao Chen and Alistair N. Boettiger and Jeffrey R. Moffitt and Siyuan Wang and Xiaowei Zhuang},
title = {Spatially resolved, highly multiplexed RNA profiling in single cells},
journal = {Science},
year = {2015},
doi = {10.1126/science.aaa6090},
url = {https://doi.org/10.1126/science.aaa6090}
}