RNA targeting with CRISPR-Cas13

Omar O. Abudayyeh, Jonathan S. Gootenberg, Patrick Essletzbichler, Feng Zhang · Full author list: Omar O. Abudayyeh, Jonathan S. Gootenberg, Patrick Essletzbichler, Shuo Han, Julia Joung, Joseph J. Belanto, Vanessa Verdine, David B. T. Cox, Max J. Kellner, Aviv Regev, Eric S. Lander, Daniel F. Voytas, Alice Y. Ting, Feng Zhang.

Published 4 October 2017 · Nature · Journal article

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Summary

This study characterized the class 2 type VI CRISPR effector Cas13a (formerly C2c2) as a programmable RNA-targeting tool in mammalian and plant cells. A catalytically inactive Cas13a (dCas13a) was used for RNA binding while active Cas13a enabled efficient, specific knockdown of endogenous transcripts. The authors showed RNA knockdown comparable to or more specific than RNA interference and demonstrated applications such as transcript tracking and splicing modulation.

Key findings

Established Cas13a (C2c2) as a programmable RNA-targeting and knockdown platform in eukaryotic cells
Achieved efficient, highly specific transcript knockdown with fewer off-targets than RNA interference
Used catalytically dead Cas13a for RNA binding, enabling transcript tracking and splicing manipulation

Subjects & keywords

Biology & Genetics cas13 rna targeting crispr rna knockdown rna editing

Cite this paper

APA

Omar O. Abudayyeh, Jonathan S. Gootenberg, Patrick Essletzbichler, & Feng Zhang [Full author list: Omar O. Abudayyeh, Jonathan S. Gootenberg, Patrick Essletzbichler, Shuo Han, Julia Joung, Joseph J. Belanto, Vanessa Verdine, David B. T. Cox, Max J. Kellner, Aviv Regev, Eric S. Lander, Daniel F. Voytas, Alice Y. Ting, Feng Zhang.] (2017). RNA targeting with CRISPR-Cas13. Nature. https://doi.org/10.1038/nature24049

BibTeX

@article{abudayyeh2017targeting,
  author    = {Omar O. Abudayyeh and Jonathan S. Gootenberg and Patrick Essletzbichler and Feng Zhang and {Full author list: Omar O. Abudayyeh, Jonathan S. Gootenberg, Patrick Essletzbichler, Shuo Han, Julia Joung, Joseph J. Belanto, Vanessa Verdine, David B. T. Cox, Max J. Kellner, Aviv Regev, Eric S. Lander, Daniel F. Voytas, Alice Y. Ting, Feng Zhang.}},
  title     = {RNA targeting with CRISPR-Cas13},
  journal   = {Nature},
  year      = {2017},
  doi       = {10.1038/nature24049},
  url       = {https://doi.org/10.1038/nature24049}
}

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